Involved in CQA monitoring? Looking for reliable HPLC methods suitable for the various aspects of this work? If so, the 'Critical Quality Attributes: Applications Compendium' is what you're looking for.
This application from Agilent describes the 2D-LC analysis of complex peptones. Active solvent modulation proved to be valuable to maintain chromatographic performance and avoid breakthrough in the second dimension.
Phosphorylated compounds such as nucleotides are known to interact with stainless steel components leading to recovery issues in HPLC and LC/MS. Instrument and column inertness ensure successful analysis. Discover more about the bio-inert...