This application note will teach you how to perform simple and rapid high-throughput analysis of biotherapeutics using different chromatographic techniques at one time.
To demonstrate the flexibility of the newly developed Thermo Scientific™ Vanquish™ Flex Duo UHPLC system for Dual LC. This study focused on the ability of the Dual LC workflow to employ two different chromatographic mechanisms at one time, without compromising data reproducibility and the quality of chromatography.
Although standard UHPLC systems can analyze samples simply and rapidly, typically only one stationary phase can be used at any given time, although there are multiple features to monitor and each requires its own method. As a result, analysts are limited regarding the number of injections that a chromatography system can perform, leading to less efficient sample analysis and additional costs. The Vanquish Flex Duo system for Dual LC can address these problems using two simultaneous LC channels for the same sample.
Full details of the experimental conditions can be found by downloading the application note.
Results and Discussion
To evaluate the flexibility of the Vanquish Flex Duo system for Dual LC, two separate studies were conducted, each employing two different stationary phases. The data from these two flexibility studies show excellent reproducibility of the instrument and outstanding performances on both channels for the two simultaneous analyses, allowing time savings and cost reduction. The Vanquish Flex Duo system for Dual LC has shown to be an easy-to-use chromatography system for the analysis of biotherapeutics. This Dual LC workflow provides the possibility to analyze biotherapeutic samples using different chromatographic techniques at one time with no compromise on the quality of analysis, which is highly
advantageous in a QC environment.
- The Vanquish Flex Duo system for Dual LC provides simple and rapid high-throughput analysis of
- Analysis of monoclonal antibodies gives excellent quality data on both chromatographic channels with high confidence in results. Excellent reproducibility with low % RSD values for a number of analytical parameters was obtained for both chromatographic channels.