Bioclinical Analysis

Solutions for Oligonucleotide Drug Analysis

Separation Science, in collaboration with Shimadzu, offers a series of application notes providing solutions for oligonucleotide drug analysis. Click on the button below to download your personal copies.

Oligonucleotide Drug Analysis


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Thermal Stability Analysis of Nucleic Acid Drugs by TMSPC™-8 Tm Analysis System
Shimadzu_App1Thermal stability analysis (Tm analysis) of a nucleic acid was conducted using a Shimadzu UV-1900i ultraviolet-visible (UV-Vis) spectrophotometer and TMSPC-8 Tm analysis system. This system provides a simple method to calculate the Tm value by measuring the absorbance transition at 260 nm at increasing temperatures.

Learning outcome:
Discover how the TMSPC-8 Tm analysis system can simplify thermal stability analysis of nucleotides.



Synthesis Confirmation for Nucleic Acid Medicines - Rapid Sequence Confirmation Using a MALDI-TOF Mass Spectrometer
Shimadzu_App2This application shows how the molecular weight and base sequence of a synthetic nucleic acid can be determined using MALDI-TOF mass spectrometry.

Learning Outcome:
See how Shimadzu’s benchtop MALDI-8020 mass spectrometer makes it easy to confirm the molecular weight and base sequence of nucleic acids.


Verification of Quantitativity and Confirmation of Molecular Weight of Oligonucleotide Therapeutics Using LCMS™-8060
Shimadzu_App3Here we introduce the analysis of a 20 base 2’-MOE modified oligonucleotide using an LCMS-8060 triple quadrupole mass spectrometer. A calibration curve for 1 to 300 ng/mL was obtained, and the molecular weight was confirmed within a small error range by deconvolution function from the scan data.

Learning Outcome:
Learn how Shimadzu’s LCMS-8060 can be used to determine the molecular weight of synthetic oligonucleotides.



Determination of Molecular Mass and Quantification of Oligonucleotide Therapeutics Using Quadrupole Time-of-Flight Mass Spectrometer LCMS™-9030
Shimadzu_App4This application shows an example analysis of a 20 base 2’-MOE modified oligonucleotide using the LCMS-9030 Q-TOF mass spectrometer. The molecular mass was determined with an error of just 0.05 ppm. A calibration curve was prepared using the MRM mode, demonstrating linearity within a range of 1–1000 ng/mL.

Learning outcome:
Learn how the accurate mass measurement of Shimadzu’s LCMS-9030 can be used to identify the structure of oligonucleotide therapeutics.

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Don't miss out on the latest COVID-19, nitrosamine analysis or LC/MS bioanalysis of antibody drugs applications. Click below for PDFs.

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Click here to access the LC/MS bioanalysis of antibody drug app notes >>

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