Bioclinical Analysis Blog

Methods for mAb and ADC analysis

Separation Science, in collaboration with Agilent, has created an educational resource including application notes outlining methods for monoclonal antibody and antibody-drug conjugate analysis. Content in this valuable learning resource includes:

High-Resolution, High-Throughput Size Exclusion Chromatography Analysis of Monoclonal
Antibodies
This application note demonstrates the use of the Agilent AdvanceBio SEC 200 Å 1.9 μm column for high-resolution and high-throughput size exclusion chromatography (SEC) analysis of a monoclonal antibody (mAb). The optimized sub-2 μm particle enables faster separations and uncompromised high resolution for accurate quantitation.

Size Exclusion Chromatography Analysis of a Monoclonal Antibody and Antibody Drug Conjugate
This application note compares SEC analysis of monoclonal antibodies (mAbs) and antibody drug conjugates (ADCs) with 2 μm and sub-2 μm SEC columns from different vendors. The Agilent AdvanceBio SEC 200 Å 1.9 μm column provides a unique advantage for high-resolution separation of mAb and ADC.

An AdvanceBio HIC Column for Drug-to-Antibody Ratio Analysis of Antibody Drug Conjugates
This application note describes the characteristics of the AdvanceBio HIC column and its use in the analysis of antibody drug conjugates (ADCs). A benefit of HIC comes from the mild conditions used for elution, which ensures that the biomolecule remains in an undenatured state.

A Comprehensive Approach for Monoclonal Antibody N-linked Glycan Analysis from Sample Preparation to Data Analysis
This study demonstrates how to increase sample throughput for glycan characterization workflows using the Agilent AssayMAP Bravo liquid handling platform.

Streamlined Workflows for N-Glycan Analysis of Biotherapeutics Using Agilent AdvanceBio Gly-X InstantPC and 2-AB Express Sample Preparation with LC/FLD/MS
The workflows described here use the Agilent AdvanceBio Gly-X with InstantPC and Gly-X 2-AB Express kits (formerly ProZyme) for the release of N-glycans using PNGase F followed by instant glycosylamine labeling with InstantPC or reductive amination labeling with 2-AB Express, respectively.

This 36-page free-access PDF is available from the 'Advances in Biomolecule Analysis' Learning Hub, which you can visit using the button below...

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