Would you like to discover an effective method for characterizing protein-nucleic acid interactions? Would you be interested in quantifying these interactions and gain a better understanding of how to control their biomolecular mechanisms? If so, this presentation from Separation Science, in collaboration with Wyatt Technology, will help you answer a few questions.
Harnessing the interactions between DNA, RNA, and proteins holds much promise for detecting biomarkers, diagnosing disease, and improving cancer-targeting therapeutics. Quantifying these interactions is essential for understanding and controlling their biomolecular mechanisms. Multi-angle light scattering (MALS) is a powerful tool for directly measuring molar masses of proteins, nucleic acids, and complexes in solution without fluorescent or radio labeling.
What is covered?
In this presentation, an expert will present two complementary light-scattering techniques for determining the stoichiometry and affinity of interactions between proteins and nucleic acids:
- Size-exclusion chromatography coupled with MALS, ultraviolet, and differential refractive index detection (SEC–MALS) analyzes each of the species present in a solution of macromolecules.
- Composition-gradient MALS (CG–MALS) quantifies the binding affinity and stoichiometry of biomolecular complexes, including multi-step reactions, label-free, and immobilization-free.
Application examples focus on protein-DNA and protein-RNA complexes.
Sophia Kenrick received her BSE in Chemical Engineering from Arizona State University and Ph.D. in Chemical Engineering from the University of California, Santa Barbara. In her graduate work, Sophia utilized a variety of biophysical techniques, including surface plasmon resonance and quantitative flow cytometry, for characterizing combinatorial protein-binding ligands.
Sophia joined Wyatt in 2010 and is a member of the Analytical Services team. She supports multiple applications for Wyatt instrumentation, especially in the field of molecular recognition and biomolecular interactions. As Dean of LSU, she facilitates the training course for all Wyatt light scattering users.