Separation Science

Announcing PREP 2018, the 31st International Symposium on Preparative and Process Chromatography

PREP 2018 takes place 8-11 July this year in the Baltimore Inner Harbor - find out more about what's going on and why you should think about attending.

HPLC Column Selection Guide to Help You Achieve the Best Separation

Dr Christian Hirsch from Shodex has compiled a comprehensive HPLC column selection guide to help you select the right HPLC column for your application and achieve the best separation out of your HPLC measurements.

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Leading the Way to Ensure the Separation Sciences Remain a Powerful Analytical Tool

Mark your calendar to attend HPLC 2018 on July 29-August 2, 2018 in Washington, DC USA. The HPLC conference will celebrate its 47th anniversary in 2018, and has grown to become the largest and most visible international conference series dedicated...

Shimadzu Launches i-Series Plus Integrated HPLC System

Shimadzu has launched its i-Series Plus integrated HPLC system that covers conventional to ultra-fast LC analysis. The launch coincides with the company's 50th anniversary of its presence in Europe.

Find out more about how iSeries Plus can improve your analytical productivity >>

Separation Science 2017 - don't miss this unique learning experience

Separation Science 2017 is the latest instalment of this highly successful conference, and will focus upon key disciplines within chromatography, mass spectrometry and related techniques, providing attendees with a unique learning opportunity...

The Case of the Unintentional Ion-Pairing Reagent

Shifting retention times within a sample batch can be a real source of headaches in the routine HPLC laboratory. Previously we talked about the kind of retention drift experienced by some HPLC methods for the first few injections. This often is...

Why do retention times drift for the first few injections of an HPLC method?

How many of you have experienced this? You think you’ve fully equilibrated the HPLC column by running mobile phase through it for 30 min, but for the first 3 or 4 injections, the retention times drift a little, finally settling down after half a...

Does a Guard Column Make Sense?

My short answer: Yes!... and No!... and Maybe. A guard column is one of those HPLC accessories that is a bit of a mixed grill – it can be a good thing or a not-so-good thing. Let’s look at some of the different aspects of guard column use to see...

Internal Standards #2: What Makes a Good Internal Standard?

In a previous blog we took a brief look at why we might decide to use an internal standard and how a calibration plot is constructed and used in HPLC analysis. This time, we’ll consider some properties that are necessary when choosing a compound...

Internal Standards #1: How Does It Work?

In the next few blogs in this HPLC Solutions serieswe’ll look at several aspects of the use of internal standards (IS) in HPLC and LC-MS applications. The topic of this discussion is how an internal standard is used in quantitative analysis.

Universal Analytical Method Development for Various HPLC Systems

Agilent Technologies has produced an application note describing a combined approach to analytical method development and method transfer by direct emulation of different target HPLC systems. The proposed workflow presents a general approach to...

Column Protection #1: Filtration or Centrifugation?

It is easy to take knowledge for granted, especially when you’ve been involved in a field for a long time, as I have with HPLC. One of those areas is about how to protect the column. In the training courses we teach, Tom Jupille and I jokingly...

     

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